Retatrutide Explained: Structure, Mass and How to Verify a Batch
Retatrutide (LY3437943) is a synthetic peptide of 39 amino acids that activates three receptors: GIP, GLP-1 and glucagon. It carries a C20 fatty diacid side chain and has a molecular mass of about 4,731.3 Da. This guide explains its structure, what the literature covers and how to verify a batch.
Key facts
- Type: Synthetic 39-amino-acid peptide; triple agonist of the GIP, GLP-1 and glucagon receptors
- Sequence: 39 residues with two Aib residues, one α-methyl-leucine and a C20 fatty diacid on lysine 17; C-terminal amide
- Molecular formula: C221H342N46O68
- Molecular mass: about 4,731.3 Da (free peptide)
- CAS number: 2381089-83-2
- Supplied as: lyophilised powder in a sealed vial
What is retatrutide
Retatrutide is a single peptide chain designed to act on three receptors at once. Three residues are not found in natural proteins: two α-aminoisobutyric acid (Aib) residues and one α-methyl-leucine. A 20-carbon fatty diacid is attached to lysine 17 through a short linker; it lets the peptide bind to albumin. The research material is made by chemical synthesis.
What the scientific literature covers
The compound was first described in 2022. Published work spans cell assays, animal models and clinical trials. Laboratory topics include:
- Activation of GIP, GLP-1 and glucagon receptors in cell-based cAMP assays
- How the fatty diacid side chain affects albumin binding and half-life
- Energy expenditure and body weight in diet-induced obese mice
- Glucose regulation in rodent models
These are laboratory findings. CellMind supplies Retatrutide for research purposes only, not for human use.
How identity and purity are verified
Retatrutide is long and carries a fatty side chain, so it behaves differently from short peptides: it elutes late on reversed-phase HPLC and can stick to surfaces. HPLC separates the target peptide from shortened and oxidised forms. Mass spectrometry confirms the mass of about 4,731.3 Da, which also tells it apart from tirzepatide (about 4,813.5 Da). Both results must appear on the certificate of analysis (CoA) for the specific batch. Our CoA guide explains how to read one.
Checklist before buying Retatrutide
- A CoA for the specific batch
- HPLC purity stated as a number, above 99%
- Mass of about 4,731 Da confirmed on the report
- A clearly stated amount per vial (10 mg)
- A batch number on the vial that matches the report
Common questions about Retatrutide
How is retatrutide different from tirzepatide?
Retatrutide activates three receptors (GIP, GLP-1 and glucagon); tirzepatide activates two (GIP and GLP-1). Both have 39 amino acids and a C20 fatty diacid, but their sequences and masses differ: about 4,731.3 Da against about 4,813.5 Da.
What mass should a retatrutide report show?
About 4,731.3 Da for the free peptide. Salt forms weigh more, so the report should state which form was measured.
What is the difference between the 10 mg and 30 mg vials?
The compound is identical; only the amount per vial differs. The CoA should state the amount for the vial size you ordered.
Storage and handling
Keep lyophilised vials in a cool, dark place. After reconstitution the solution should be refrigerated and used within the planned study period. Avoid repeated temperature changes: they are the most common cause of degradation that nobody notices until results stop matching.
CellMind Retatrutide 10mg
- 10 mg of lyophilised powder per vial, sealed under inert conditions
- HPLC-tested by Janoshik Analytical, purity above 99%
- A CoA for the specific batch with every order
Product page: Retatrutide 10mg. Ordering and delivery: Where to buy Retatrutide. Reports for current batches: Lab reports and CoA.
Sources
Research purposes only.